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Carbohydrate Metabolism by Willis A. Wood (Eds.)

By Willis A. Wood (Eds.)

The severely acclaimed laboratory usual, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. considering the fact that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this present day - actually an important booklet for researchers in all fields of existence sciences

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Two grams 2,4"dibromoacetophenone (Eastman Organic Chemicals) is recrystallized from 8-10 ml ethanol before use. Addition of 100 mg Norit A to the boiling alcoholic solution and filtration on a sintered glass funnel results in a colorless solution. Upon cooling in an ice bath, pure white crystals are obtained which are washed with a small volume of pentane. Silica gel G for thin layer chromatography, according to Stahl, can be obtained from Brinkmann Instruments Inc. and silica gel 200 mesh from Davison Chemical Company.

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Anderson and D. P. Allison, J. Biol. Chem. 240, 2367 (1965). 17D. P. Burma and B. L. Horecker, J. Biol. Chem. 231, 1053 (1958). 18S. Mitsuhashi and J. 0. Lampen, J. Biol. Chem. 204, 1011 (1953). 19K. Yamanaka, Agr. Biol. Chem. 26, 175 (1962). 2, R. L. Anderson and W. A. Wood, J. Biol. Chem. 237~ 296 (1962). [9] KETOPENTOSE 5-PHOSPHATES 43 The preparation of D-xylulose 5-phosphate with the purified D-xylulokinase prepared from A e r o b a c t e r aerogenes 6 is described below. Other pentulose 5-phosphates can be prepared by phosphorylating the pentulose with the appropriate kinase.

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